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| 名称 | M-07e (人巨细胞白血病细胞) (STR鉴定正确) |
| 别称 | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
| 背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available |
| 种属 | 人类 |
| 年龄（性别） | 女性，6个月 |
| 组织来源 | 外周血 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 白血病细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | RPMI-1640＋10% FBS＋8ng/mL GM-CSF+1% P/S |
| 推荐传代比例 | 5×10^5-1×10^6cells/mL |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~40小时 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 抗原表达情况 | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR - |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 保藏机构 | DSMZ; ACC-104 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 9,10 |
| D2S1338 | 19,25 |
| D3S1358 | 16,19 |
| D5S818 | 11 |
| D7S820 | 11 |
| D8S1179 | 13,16 |
| D13S317 | 10,11 |
| D16S539 | 11 |
| D18S51 | 15,20 |
| D19S433 | 14,15 |
| D21S11 | 30,32.2 |
| FGA | 18,22 |
| PentaD | 9 |
| PentaE | 12,14 |
| TH01 | 6,8 |
| TPOX | 8 |
| vWA | 16,18 |
| D6S1043 | 13,19 |
| D12S391 | 23,24 |
| D2S441 | 14 |
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